Journal: Scientific Reports

Article Title: α-Lipoic acid induces Endoplasmic Reticulum stress-mediated apoptosis in hepatoma cells

doi: 10.1038/s41598-020-64004-5

Figure Lengend Snippet: α-LA induces ER stress in hepatoma cells. ( A ) Western analysis of key players in UPR after treatment of HepG2 with 500 µM α-LA from 6 up to 48 hours. Albumin was used as loading control. For densitometric analysis protein expression was normalized to Albumin expression and values (reported over each band) have been expressed as fold change respect to control. Each lane represents a pool of three individual samples. ( B ) Schematic representation of α-LA-mediated apoptosis in hepatoma cells. Western blot images ( A ) have been cropped for clarity with full blot presented in Supplementary Fig. .

Article Snippet: The rat hepatoma cell line, FaO, and the hepatocarcinoma cell line, HepG2, were supplied by Interlab Cell Line Collection (Servizio Biotecnologie, IST, Genova, Italy), and maintained, respectively, in Dulbecco’s medium (DMEM plus Glutamax I) (Invitrogen) and supplemented with penicillin, streptomycin and 10% heat-inactivated fetal calf-serum (FCS) (Invitrogen) in a humidified atmosphere of 5% CO 2 /95% air, at 37 °C. α-Lipoic Acid (α-LA) and Thapsigargin (TG) were purchased from Sigma (Sigma-Aldrich, Milano, Italy). α-LA, dissolved in sodium hydroxide NaOH 1 N and neutralized in medium, and TG dissolved in DMSO, were added to the culture media to the final concentrations specified in the text.

Techniques: Western Blot, Control, Expressing

Journal: PLoS ONE

Article Title: Cellular Trafficking of Thymosin Beta-4 in HEPG2 Cells Following Serum Starvation

doi: 10.1371/journal.pone.0067999

Figure Lengend Snippet: Tβ 4 immunoreactivity in HepG2 cells cultured for 48 h with serum (A and B, magnification 1000×) and without serum (C and D, magnification 1000×). Inserts in A and in C represent one particular of the intranuclear reactivity of the respective pictures (magnification 1000×).

Article Snippet: Commercial human cell line HepG2 (ICLC HTL95005), were obtained from the Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova).

Techniques: Cell Culture

Journal: PLoS ONE

Article Title: Cellular Trafficking of Thymosin Beta-4 in HEPG2 Cells Following Serum Starvation

doi: 10.1371/journal.pone.0067999

Figure Lengend Snippet: Tβ 4 immunoreactivity in HepG2 cells cultured for 72 h with serum (A and B, magnification 1000×) and without serum (C and D, magnification respectively 400 and 1000×). Inserts in A and in C represent one particular of the intranuclear reactivity of the respective pictures (magnification 1000×).

Article Snippet: Commercial human cell line HepG2 (ICLC HTL95005), were obtained from the Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova).

Techniques: Cell Culture

Journal: PLoS ONE

Article Title: Cellular Trafficking of Thymosin Beta-4 in HEPG2 Cells Following Serum Starvation

doi: 10.1371/journal.pone.0067999

Figure Lengend Snippet: Tβ 4 immunoreactivity in HepG2 cells cultured for 84 h with serum (A and B, magnification 1000×) and without serum (C and D, magnification 1000×). Inserts in A and in C represent one particular of the intranuclear reactivity of the respective pictures (magnification 1000×).

Article Snippet: Commercial human cell line HepG2 (ICLC HTL95005), were obtained from the Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova).

Techniques: Cell Culture

Journal: PLoS ONE

Article Title: Cellular Trafficking of Thymosin Beta-4 in HEPG2 Cells Following Serum Starvation

doi: 10.1371/journal.pone.0067999

Figure Lengend Snippet: Tβ 4 immunoreactivity in HepG2 cells cultured for 84 h without serum and shifted in complete medium for 24 h (A and B, magnification 400×) and for 48 h (C and D, magnification 400×). Inserts in A and in C represent one particular of the intranuclear reactivity of the respective pictures (magnification 1000×).

Article Snippet: Commercial human cell line HepG2 (ICLC HTL95005), were obtained from the Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova).

Techniques: Cell Culture

Journal: Biomaterials

Article Title: Tissue-Specific Extracellular Matrix Coatings Promote Cell Proliferation and Maintain Cell Phenotypes

doi: 10.1016/j.biomaterials.2009.04.005

Figure Lengend Snippet: Effect of various tissue-specific ECM coatings (0.05mg/ml) on cell adhesion

Article Snippet: An established human hepatocarcinoma cell line, HepG2, was also used for this study (GeneTex.

Techniques:

Journal: Biomaterials

Article Title: Tissue-Specific Extracellular Matrix Coatings Promote Cell Proliferation and Maintain Cell Phenotypes

doi: 10.1016/j.biomaterials.2009.04.005

Figure Lengend Snippet: HepG2 cells were cultured on different tissue-specific ECM coatings for 14 days before protein lysates were prepared. Proteins were electrophoresed and transferred to a membrane, which was probed with antibodies to HAS, albumin and β-actin (loading control). Higher expression of liver-specific proteins are visible when cells are cultured on ECM versus control (no ECM coating) plates.

Article Snippet: An established human hepatocarcinoma cell line, HepG2, was also used for this study (GeneTex.

Techniques: Cell Culture, Membrane, Control, Expressing